Journal: Frontiers in Endocrinology

Article Title: The PTP1B inhibitor MSI-1436 ameliorates liver insulin sensitivity by modulating autophagy, ER stress and systemic inflammation in Equine metabolic syndrome affected horses

doi: 10.3389/fendo.2023.1149610

Figure Lengend Snippet: List of used ELISA assays kits.

Article Snippet: Horse Leptin , BT LAB , E0047Ho.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Immunology

Article Title: Ultraviolet B Inhibits IL-17A/TNF-α-Stimulated Activation of Human Dermal Fibroblasts by Decreasing the Expression of IL-17RA and IL-17RC on Fibroblasts

doi: 10.3389/fimmu.2017.00091

Figure Lengend Snippet: Real-time quantitative PCR analyses of HDFs stimulated with IL-17A (100 ng/ml) and TNF-α (10 ng/ml) alone or together for 24 h. The results demonstrate that both IL-17A and TNF-α induce significant increases in IL-6, IL-8, and CXCL-1 mRNA expression and IL-17A/TNF-α stimulation have additive or synergistic effect on IL-6, IL-8, and CXCL-1 mRNA expression . Additionally, IL-17A induces the mRNA expression of TNFR-2 and TNF-α induces IL-17RA and IL-17RC expression on HDFs. The data (fold change) are from one representative experiment performed in triplicate, repeated three times with similar results. HDFs, human dermal fibroblasts. Statistical significance indicated: * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: Recombinant human IL-17A (100 ng/ml), TNF-α (10 ng/ml), and TGF-β1 (2 ng/ml) were obtained from PEPROTECH (Rocky Hill, NJ, USA), and P144 (2 μg/ml) was purchased from MedChem Express (Monmouth Junction, NJ, USA).

Techniques: Real-time Polymerase Chain Reaction, Expressing

Journal: Frontiers in Immunology

Article Title: Ultraviolet B Inhibits IL-17A/TNF-α-Stimulated Activation of Human Dermal Fibroblasts by Decreasing the Expression of IL-17RA and IL-17RC on Fibroblasts

doi: 10.3389/fimmu.2017.00091

Figure Lengend Snippet: Real-time quantitative PCR analyses of HDFs treated with IL-17A (100 ng/ml)/TNF-α (10 ng/ml), UVB (30 mJ/cm 2 ), and TGF-β1 (2 ng/ml) for 24 h . (A) IL-17A/TNF-α-stimulated HDFs increase expression of IL-6, IL-8, and CXCL-1 mRNA, which are also seen in 30 mJ/cm 2 UVB-irradiated HDFs. But UVB irradiation inhibits IL-17A/TNF-α-induced IL-6, IL-8, and CXCL-1 mRNA expression of HDFs. IL-17A/TNF-α stimulation induce the expression of TNFR-2, IL-17RA, and IL-17RC mRNA. UVB irradiation upregulates the expression of TNFR-1 and TNFR-2 mRNA but downregulates IL-17RA and IL-17RC expression, and inhibits IL-17A/TNF-α-induced IL-17RA and IL-17RC mRNA expression. IL-17A/TNF-α and UVB treatment do not induce significant expression of TGF-β1 mRNA 24 h after culture, but Smad3 mRNA is upregulated in both UVB irradiation and IL-17A/TNF-α + UVB groups. (B) TGF-β1 significantly induces the Smad3 mRNA expression and downregulates the IL-17RA and IL-17RC expression in HDFs. The data (fold change) are from one representative experiment performed in triplicate, repeated three times with similar results. UVB, ultraviolet B; HDFs, human dermal fibroblasts. Statistical significance indicated: * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: Recombinant human IL-17A (100 ng/ml), TNF-α (10 ng/ml), and TGF-β1 (2 ng/ml) were obtained from PEPROTECH (Rocky Hill, NJ, USA), and P144 (2 μg/ml) was purchased from MedChem Express (Monmouth Junction, NJ, USA).

Techniques: Real-time Polymerase Chain Reaction, Expressing, Irradiation

Journal: Frontiers in Immunology

Article Title: Ultraviolet B Inhibits IL-17A/TNF-α-Stimulated Activation of Human Dermal Fibroblasts by Decreasing the Expression of IL-17RA and IL-17RC on Fibroblasts

doi: 10.3389/fimmu.2017.00091

Figure Lengend Snippet: Protein detection by using Western blot, ELISA analysis, and flow cytometry . (A) Western blot of HDFs lysates confirms mRNA expression of IL-6, IL-8, and CXCL-1 at the protein level, although shows no significant increase in IL-8 protein of UVB group compared with Control group and no significant decrease in IL-6 and CXCL-1 protein of IL-17A/TNF-α + UVB group compared with IL-17A/TNF-α group. (B) ELISA analysis of the supernatant confirms mRNA expression of IL-6, IL-8, and CXCL-1 at the protein level. (C) The expression of IL-17RA and IL-17RC mRNA is confirmed by flow cytometry for detection of the surface expression of IL-17RA and IL-17RC on HDFs. The data (fold change) of Western blot are expressed as the mean ± SD of three independent experiments. Other data are from one representative experiment performed in triplicate, repeated three times with similar results. UVB, ultraviolet B; HDFs, human dermal fibroblasts. Statistical significance indicated: * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: Recombinant human IL-17A (100 ng/ml), TNF-α (10 ng/ml), and TGF-β1 (2 ng/ml) were obtained from PEPROTECH (Rocky Hill, NJ, USA), and P144 (2 μg/ml) was purchased from MedChem Express (Monmouth Junction, NJ, USA).

Techniques: Western Blot, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Expressing, Control

Journal: Frontiers in Immunology

Article Title: Ultraviolet B Inhibits IL-17A/TNF-α-Stimulated Activation of Human Dermal Fibroblasts by Decreasing the Expression of IL-17RA and IL-17RC on Fibroblasts

doi: 10.3389/fimmu.2017.00091

Figure Lengend Snippet: Protein detection by using ELISA analysis, Western blot, and flow cytometry . (A) ELISA analysis shows significant TGF-β1 protein secretion in supernatant in both UVB and IL-17A/TNF-α + UVB groups. (B) Western blot shows the protein expression of Smad3 is significantly upregulated in both UVB and IL-17A/TNF-α + UVB groups. (C) Flow cytometry shows P144 (2 µg/ml) is able to block the inhibitory effect of UVB on the expression of IL-17RA and IL-17RC on HDFs. The data (fold change) of Western blot are expressed as the mean ± SD of three independent experiments. Other data are from one representative experiment performed in triplicate, repeated three times with similar results. UVB, ultraviolet B; HDFs, human dermal fibroblasts. Statistical significance indicated: * P < 0.05, ** P < 0.01.

Article Snippet: Recombinant human IL-17A (100 ng/ml), TNF-α (10 ng/ml), and TGF-β1 (2 ng/ml) were obtained from PEPROTECH (Rocky Hill, NJ, USA), and P144 (2 μg/ml) was purchased from MedChem Express (Monmouth Junction, NJ, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry, Expressing, Blocking Assay

Journal: bioRxiv

Article Title: A role for fibroblast and mural cell subsets in models of neuropathic pain

doi: 10.1101/2024.12.11.627455

Figure Lengend Snippet: Each dot is a well of cells. Filled circles shows the expression levels obtained from media taken from a placental pericyte line, open circles those obtained from a nerve pericyte line. Ctrl = regular pericyte media; other columns: pericytes treated for four hours with TNF (10ng/ml), IL-17 (10ng/ml), TNF+IL-17 (both at 10ng/ml), IL-4 (10ng/ml) or IFNγ (100ng/ml). The red dotted lines represent the upper and lower limits of the standard curves for each protein, i.e. the upper and lower detection limits. Shown here are 1:100 dilutions for IL-6 and NGF and 1:2 dilutions for CCL2.

Article Snippet: After 24 hours, the media were again changed to serum starved media with the addition of human recombinant cytokines (10ng/ml TNFα, BioLegend #570102, 10ng/ml IL-17, Proteintech #Hz-1113, 10ng/ml IL-4, BioLegend #574004, 100ng/ml IFNγ, BioLegend #570202).

Techniques: Expressing